FIG. 3.

Undifferentiated hESCs and SD-hESCs develop morphologically distinctive EBs. (A) Colonies derived from undifferentiated hESCs and SD-hESCs were mechanically separated and cultured to develop EBs and analyzed by phase-contrast imaging at different time points. Colonies derived from undifferentiated hESCs and SD-hESCs were morphologically distinctive with tightly packed cell clusters (indicated by arrows ↑) and distinct layers within EBs with fluid-filled and balloon-like cysts (indicated by arrowhead ▴). (B) Percentages of cystic EBs were counted at serial time points (5, 10, 15 days) after EB formation. Data are presented as mean values±SD from five independent experiments. Student's t-test: *p<0.05 and **p<0.01. Color images available online at www.liebertpub.com/scd