FIG. 6.

RT-PCR analysis of genes involved in the intracellular binding of ROL and RA in NE-4C neural stem cells and RGl cells. (A) RT-PCR analysis of CrbpI and CrabpI-II in nondifferentiated (Ø) and differentiating NE-4C cells. Differentiation was induced either by serum withdrawal (DM) or RA treatment (DM+RA). (B) RT-PCR analysis of CrbpI and CrabpI-II expression in nondifferentiated (EGF+) and differentiating (EGF−) RGl cells. DIV indicates in vitro days after the onset of induction. “−” and “+” represent the negative (water) and positive (liver) controls of the RT-PCR reactions. “M” indicates molecular weight marker.