FIG. 2.

NSCs derived from human iPSCs. (A) Human iPSCs were efficiently induced into neural spheres and mature neural cells in defined culture system. (B) iPSCs-derived neural spheres expressed Nestin. (C) iPSCs-derived neural cells were positive for GFAP or tubulin. (D) New neurons differentiated by NSCs could generate action potentials in vitro by electrophysiological recordings. (E) Human iPSCs-derived NSCs treated with SPIOs showed intracellular iron accumulation, as visualized by PB staining (blue particles). (F) Electron micrographs of SPIO-labeled NSCs revealed the presence of iron nanoparticles (the black arrow) in the cytoplasm at 1 week after culture. (G) Proliferation of SPIO-labeled (blue bars) and unlabeled NSCs (red bars) exhibited no significantly statistic difference. Values are mean±standard deviation, p>0.05. (H) Differentiation of SPIO-labeled (blue bars) and unlabeled (red bars) NSCs present no difference. Values are means±standard deviation, p>0.05. Scale bar, 50 μm.