FIG. 3.

Endothelial potential of CD34⁺CD31⁺VEC⁺ cells. CD34⁺CD31⁺VEC⁺ cells were isolated from day 10 EBs, and then cultured in the serum-free endothelial cell growth medium (SFEGM). After 4 days of culture, the cells showed hematopoietic-like colonies (A). After 6 days, the adhesive cells displayed endothelial characteristics, including typical morphology of endothelial cells (B), positive for VEC by immunohistochemistry (C) and flow cytometry analysis (D), Dil-acetylated low-density lipoprotein (Dil-Ac-LDL) uptake (E), and vascular network formation on Matrigel (F). A light phase photo was inserted to show the endothelial morphology (E).