Figure 4.

FlnA regulates Big2 expression. A, Western blot shows increased BIG2 protein levels in FLNA-deficient M2 cells compared with FLNA-replete A7 cells. B, Similarly, BIG2 levels are elevated by Western blot following FlnA knockdown by shRNAi in stably transfected Neuro2A neuroblastoma cells. C, Last, enhanced Big2 expression is found in FlnA-null progenitor cells compared with FlnA WT cells. Tubulin is used as a loading control. D, Fluorescent photomicrograph shows an overall increase in Big2 immunostaining (asterisks) in null FlnA ventricular zone. Scale bar, 20 μm.