Figure 8.

Examination of levels of monocytic populations in Cd11bCre;EP2^+/+ and Cd11bCre;EP2^lox/lox mice. Splenocytes and peripheral blood immune cells were isolated from 3-month-old mice. A, Representative plots for CD11b⁺ cells gated on CD115 and Ly6C yielding four populations of cells, including CD115⁻/Ly6C⁻ macrophages, CD115⁻/Ly6C^int-hi neutrophils, CD115^int/Ly6C^int resident monocytes, and CD115^hi-int/Ly6C^hi inflammatory monocytes in vehicle and LPS-treated mice. B, Quantification of levels of monocytic populations, including macrophages, resident monocytes, and inflammatory monocytes does not show differences between genotypes in vehicle or LPS-treated mice. Levels of neutrophils are decreased in peripheral blood with LPS, but not vehicle stimulation (*p < 0.05; n = 4 mice per group). C, Quantification of CD11b-positive microglia derived from brains of Cd11bCre;EP2^+/+ and Cd11bCre;EP2^lox/lox mice does not show differences in number (n = 5–7 mice per genotype). D, Comparison of copy number of EP2/copy number of 18S is shown for adult microglia and peritoneal macrophages from Cd11bCre;EP2^+/+ and Cd11bCre;EP2^lox/lox mice (n = 4–6 per group; p < 0.05 unpaired t test). Macrophage expression of EP2 in Cd11bCre;EP2^+/+ mice was 28-fold higher; however, the percentage reduction of expression with conditional deletion of EP2 was similar in both microglia and macrophages, and was 62.2 and 62.1%, respectively.