Figure 3.
Coculture of skeletal muscle* sensory neurons (DRG)* spinal cord neurons. All neurons were stained with the antibody against neurofilament-M (150 KD) (Red) and the myotubes were stained with the antibody against embryonic myosin heavy chain (F 1.652) (Green). Scale bar is 75 μm. A. The large sensory neurons (DRG) were stained with NF 150 (Red). The neurons are shown with white arrows. The myotubes stained with F1.652 (Green) were seen in the same field. B. The neuron process in red are noted running parallel to the myotubes (Green). C. The multi-polar motoneuron was noted in close proximity with the myotubes. In the frame multiple large sensory neurons were observed. All the neurons are pointed out by white arrows. D. A bundle of multiple processes of neurons crossed the striated, branched myotube. E. A single multi-polar motoneuron was observed to wrap around the mytotube. F. Neuron processes wrapping on the myotubes.