Table 2.
Composition of novel serum-free medium for a 500 ml sample.
| S. No. | Component(s) | Catalogue # | Company | Amount |
|---|---|---|---|---|
| 1. | L15 | Invitrogen | 11415064 | 375 ml |
| 2. | M199 | Invitrogen | 11150059 | 125 ml |
| 3. | Vitronectin | Sigma | V0132 | 50 μg |
| 4. | B27 | Invitrogen | 17504044 | 10 ml |
| 5. | Basic FGF | Invitrogen | 13256029 | 10ng/ml |
| 6. | CT1 | Cell Sciences | CRC700B | 10 μg |
| 7. | GDNF | Invitrogen | 10907012 | 10 μg |
| 8. | BDNF | Invitrogen | 10908019 | 10 μg |
| 9. | NT3 | Cell Science | CRN 500B | 10 μg |
| 10. | NT4 | Cell Science | CRN501B | 10 μg |
| 11. | Sodium Bicarbonate | Fisher | 5233500 | 0.7 g |
| 12. | Osmolarity | 320–325 mOsm | ||
| 13. | pH | 7.3 |