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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2011 Aug 5;187(5):2155–2161. doi: 10.4049/jimmunol.1003235

Figure 4.

Figure 4

Generation of Th17 cells in healthy donor PBMC cultures supplemented with burn patient sera previously treated with various mAbs. Five PBMC preparations (2 × 106 cells/ml) isolated from healthy donors (#1 to #5) were individually cultured with CAg and sera from burn patients #10, #11, #12 and #15. Before being used to cultures, burn patient sera were treated with 2.5 μg/ml of anti-IL-10, anti-IL-4, anti-CCL2 mAb or isotype control Ab (rat IgG2a) for 30 min at 4°C. Culture fluids harvested 3 days after stimulation were assayed for IL-17, and cells harvested from these cultures were analyzed for intracellular expression of IL-17 and RORγt by flow cytometry. The results obtained were combined and displayed in the figure by the mean ± SEM of the 5 experiments. ** p<0.01 vs. PBMC cultured with CAg and burn patient serum treated with isotype control Ab.