Figure 3.

CHS negatively influences the immune effects induced by formalin-inactivated H5N1 virus. Serum samples and T cells of three mice from CHS+Ag and TN+Ag groups were collected on indicated days postprimary immunization. (a) Antibody levels against the H5 subtype antigen were expressed by HI titer. (b) Ag-specific IgG, IgG1, and IgG2a were determined by ELISA. (c) T cells were isolated on day 21 and stimulated with specific antigen; cell proliferation was determined by MTS/PMS method. (d) T cells collected on day 21 were stimulated with a viral-specific antigen for 6 h in vitro, stained with different florescent antibodies, and analyzed by flow cytometry. The numbers in the box indicate the percentages of examined cytokines. Data are summarized in a bar graph to the right. Results shown are pooled from three independent experiments.