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Linearity of XA determination as assayed by the proposed spectrophotometric method. Each point represents an average of triplicate determinations. The XA stock solution (100 mg/L) was prepared by dissolving 10 mg of XA into 1.0 mL of methanol and then made up to 100 mL with XA-free pooled urine. Various concentrations of standards were then made up by diluting with XA-free urine to the desired concentrations.
