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Purification of 3DHSA-G-CSF. The supernatants containing the target proteins were purified by Cibacron Blue F3G-A Sepharose and Butyl-Sepharose 4B column, and the final purity was detected by 12% SDS-PAGE. (a) The hydrophobic chromatograph of 3DHSA-G-CSF by Butyl-Sepharose 4B column. (b) 12% SDS-PAGE, M: protein marker, Lanes 1~3: flow through, Lanes 4~6: elute.
