Table 1.
Origin and identification of rhizospheric LAB isolates.
| Geographical position | Sampling point | Source/number of soil samples | Number of isolates | Strains | Closest 16S rDNA sequence | Strains (access number) | % of sequence similarity |
|---|---|---|---|---|---|---|---|
| Northeast Tunisia | Ben Arous | R. of olive tree/03 | 4 | FS01 | Enterococcus hirae | ||
| FS02 | Lactococcus lactis | ||||||
| FS03 | Enterococcus faecalis | ||||||
| SSR of olive tree 01 | F S04 | Weissella confusa | FS04 ( KC568542) | 99 | |||
| Tunis | R. of olive tree/03 | 11 | FS07 | Lactococcus lactis | |||
| FS08 | Weissella halotolerans | FS08 (KC568554) | 99 | ||||
| FS11 | Enterococcus faecalis | FS11 (KC568559) | 99 | ||||
| FS13 | Leuconostoc mesenteroides | FS13 (KC568533) | 97 | ||||
| FS14 | Enterococcus durans | ||||||
| FS09, FS10, FS12, FS15 | Enterococcus faecium Enterococcus faecium | FS10 (KC568539) | 99 | ||||
| SSR of olive tree/01 | FS05, FS06 | ||||||
|
| |||||||
| Northwest Tunisia | Jendouba | R. of olive tree/21 | 50 | FS16, FS17, FS19, FS21, FS25, FS26, FS30, FS40, FS48, FS50, FS51, FS55, FS56, FS57, FS65 | Enterococcus faecium | FS25 (KC568541), FS19 (KC568549) FS26 (KC568553), FS65 (KC568552) |
99 |
| FS18 | Enterococcus faecalis | ||||||
| FS20 | Lactococcus lactis | ||||||
| FS24, FS37, FS38, FS41 | Pediococcus pentosaceus | FS24 (KC568551), FS37 (KC568550) | 99 | ||||
| FS46 | Pediococcus acidilactici | FS46 (KC568555) | 98 | ||||
| FS29, FS32, FS42, FS49 | Enterococuus durans | FS29 (KC568547) | 98 | ||||
| FS22, FS31 | Lactococcus garviae | FS31 (KC568548) | 99 | ||||
| FS33, FS34, FS62 | Lactobacillus sakei | FS33 (KC568535) | 100 | ||||
| FS35, FS59 | Lactobacillus plantarum | FS35 (KC568557) | 99 | ||||
| FS39, FS43, | Enterococcus hirae | FS39 (KC568531) | 99 | ||||
| FS45, FS60, FS64 | Weissella paramesenteroides | FS64 (KC568556) | 99 | ||||
| FS58 | Weissella halotolerans | FS58 (KC568532) | 99 | ||||
| FS36, FS44, FS52, FS53, FS54, FS61, FS63 | Weissella confusa | FS61 (KC568543), FS53 (KC568544), FS52 (KC568545), | 99 | ||||
| SSR of olive tree/04 | FS23, FS27, FS28, | Weissella confusa | FS 27 (KC568537) | 99 | |||
| FS47 | Enterococcus faecium | ||||||
|
| |||||||
| The middle Tunisia | Kairouan | R. of olive tree/03 | 9 | FS69 | Lactobacillus sakei | ||
| FS66 | Weissella confusa | FS66 (KC568540) | 99 | ||||
| FS73 | Pediococcus pentosaceus | ||||||
| FS67, FS68, FS70, FS71, FS72, FS74 | Enterococcus faecium | ||||||
|
| |||||||
| South Tunisia | Gafsa | R. of olive tree/02 | 3 | FS76 | Weissella confusa | ||
| FS75 | Lactococcus lactis | FS75 (KC568534) | 99 | ||||
| SSR/01 | FS77 | Weissella confusa | |||||
| Kebili | R. of olive tree/06 | 23 | FS81, FS86, FS100 | Enterococcus hirae | |||
| FS78, FS79, FS80, FS82, FS83, FS84, FS85, FS87, FS88 | Enterococcus faecium | FS88 (KC568538) | 99 | ||||
| FS89, FS90, FS91, FS92, FS93, FS94, FS95, FS98, FS99 | |||||||
| SSR/01 | FS96, FS97 | Enterococcus faecium | |||||
| Mednine | R. of olive tree/03 | 7 | FS101, FS102, FS103, FS105, FS106, FS107 | Enterococcus faecium | |||
| FS104 | Pediococcus pentosaceus | ||||||
| R. of truffle/04 | 12 | FS111 | Pediococcus pentosaeus | FS111 (KC568560) | 98 | ||
| (Terfezia boudieri/Pichoa) | FS119 | Lactobacillus plantarum | FS119 (KC568558) | 99 | |||
| FS110 | Enterococcus durans | ||||||
| FS112 | Enterococccus hirae | ||||||
| FS108, FS109, FS113, FS114, FS115, FS116, FS117, FS118 | Enterococcus faecium | FS118 ( KC568536) | 99 | ||||
R: Rhizosphere samples; SSR: soil surrounding rhizosphere; the underlined strains refer to LAB isolated from the SSR.