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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Cell Immunol. 2012 Apr 11;276(0):67–74. doi: 10.1016/j.cellimm.2012.03.010

Fig. 2. Effect of CTX treatment on the relative and absolute numbers of CD11c+CD11b− and CD11c+CD11b+ cells.

Fig. 2

C57BL/6 mice were injected i.p. with PBS or CTX (4 mg/mouse). The average of the data from PBS-treated mice (control group) is depicted as day 0 in the figure. At the indicated time points, mice were bled to collect PBL and then sacrificed to harvest spleen and BM. Leucocytes were counted, stained with mAbs against the indicated cell markers, and then analyzed by flow cytometry. The total leukocytes were gated after exclusion of dead cells and from which the frequency of CD11c+CD11b+ (R1) and CD11c+CD11b− (R2) was analyzed in the blood as a representative dot plot analysis as shown in (A). The relative and absolute numbers in PBL (B), spleen (C), and BM (D) are depicted. The absolute numbers were calculated as (total cell number × %) /100. Data represent the mean ± SD (n = 4).