Table 1.
Cloning |
||||||
---|---|---|---|---|---|---|
Everglades | Suwannee | Central Florida | North Carolina | Gulf–Atlantic | Total | |
Clones collected and sequenced | 480 | |||||
Clones containing an L1 element | 380 | |||||
Total sequences mapped to database | 265 | |||||
Number of different L1AC18 inserts | ||||||
Flanking sequences located in database | 55 | 23 | 43 | 22 | 29 | 172 |
Insertion sites occupied in database | 42 | 20 | 37 | 17 | 21 | 137 |
Insertion sites empty in database | 13 | 3 | 6 | 5 | 8 | 35 |
Tested by PCR | 10 | 3 | 5 | 4 | 6 | 28 |
Proportion <50% polymorphism | 80% | 63% | 83% | 100% | 0 | |
No. FL inserts | 0 | 2 | 2 | 1 | 4 | 9 |
Proportion FL >50% | – | 0 | 0 | 0 | 100% | |
Number of different L1AC20 inserts | ||||||
Flanking sequences located in database | 18 | 12 | 34 | 14 | 15 | 93 |
Insertion sites occupied in database | 12 | 9 | 26 | 14 | 12 | 73 |
Insertion sites empty in database | 6 | 3 | 6 | 0 | 3 | 18 |
Tested by PCR | 6 | 2 | 5 | – | 2 | 15 |
Proportion <50% polymorphism | 100% | 50% | 100% | – | 50% | |
No. FL inserts | 0 | 2 | 0 | 0 | 0 | 2 |
Proportion FL >50% (%) | – | 50 | – | – | – |