Fig. 3.

Non-polar lipid composition in P. pastoris strains deleted of DGA1, LRO and ARE2.

Cells were grown at 30 °C on YPD or YPO media, respectively, to the early stationary phase. Total cell lipids were extracted and analyzed by TLC as described in the methods section.

A: TG content in deletion strains grown on glucose (gray bars) and oleate (white bars). B: SE content in deletion strains grown on glucose (gray bars) and oleate (white bars). Data are mean values of at least three independent experiments. Error bars indicate the standard deviation. Significance was calculated by Student's t-test (one tailed, unpaired). p values are as follows: * < 0.05; ** < 0.01; *** < 0.005.