Fig. 2.

Phosphorylation of the cytosolic regions of GIRK4 by PKA in-vitro.

A.: upper panel: Autoradiogram showing ³²P incorporation of the GIRK4 C- and N-terminal fusion proteins following incubation with PKA-cs and ATP-γ-³²P. Lower panel: Coomassie stain of the electrophoresis gel shown in upper panel.

B: Statistical analysis of ³²P incorporation into N- and C-terminus. The number of individual phosphorylation experiments is shown in parenthesis above each bar. ***: the mean value deviates statistically significant from GST at the p < 0.001 level.