Fig. 2.

mdlc RNAi results in repression of Pros in postmitotic neurons. (A-F) Type I neuroblasts. (A-C) Newly born GMCs marked by a 4-hour pulse of EdU and immediately stained; both wt GMCs (A) and mdlc RNAi GMCs (B) are Pros⁺, as quantified in C. n=304 EdU⁺ cells in wt and n=99 EdU⁺ cells in mdlc RNAi, 100% of which were Pros⁺. (D-F) Mature neurons marked by a 4-hour pulse of EdU followed by a 36-hour EdU-free chase; wt neurons are Pros⁺ (D) whereas mdlc RNAi neurons (E) fail to maintain Pros, as quantified in F. n=1016 EdU⁺ cells in wt, 79% of which were Pros⁺; n=438 EdU⁺ cells in mdlc RNAi brains, 44% of which were Pros⁺. *P<0.01; error bars indicate s.d. (G-J) Type II neuroblasts. (G,H) Newly born GMCs marked by a 4-hour pulse of EdU and immediately stained; both wt GMCs (G) and mdlc RNAi GMCs (H) are Pros⁺. (I,J) Mature neurons marked by a 4-hour pulse of EdU followed by a 36-hour EdU-free chase; wt neurons are Pros⁺ (I) whereas mdlc RNAi neurons (J) fail to maintain Pros. All yellow arrowheads mark EdU⁺ Pros⁺ cells; all white arrowheads mark EdU⁺ Pros^- cells. Scale bars: 10 μm.