Fig. 3.

SAA and SAP were present in amyloid deposits of AKU cartilage and synovia and both co-localized with melanin. A) SAA and SAP deposition in AKU cartilage and synovial specimens was detected by dual immunofluorescence technique. AKU cartilage and synovia showed high levels of SAA deposit superimposing SAP deposits. Positive staining for SAA and SAP was particularly intense in correspondence of ochronotic shards. Bar: 75 μm; B) AKU cartilage sections were dual-stained using antibodies specific for SAA SAP and compared to melanin fluorescence, resulting in a perfect co-localization of amyloid deposits and pigmented areas. Cartilage specimen was from Patient 6 and synovia specimen was from Patient 1. DIC: differential interference contrast. Bar: 150 μm. Representative images from a triplicate set are shown.