Fig. 3.

A strain containing cytochrome bd-I as sole oxidase is more resistant to growth inhibition by CORM-3 than are strains expressing other oxidases. CORM-3 (12.5 μM) was added at early exponential phase to cultures of E. coli as follows: (A) parent strain; (B) expressing cytochrome bd-I only; (C) expressing cytochrome bd-II only and (D) expressing cytochrome bo′ only. Data in (A)–(D) show growth in the absence of any addition (closed symbols) and presence (open symbols) of CORM-3. Points are means and standard deviations of three biological replicates. (E) Shows the doubling times of these strains in the 2 h following CORM-3 addition, before (black bars) and after (grey bars) the addition of CORM-3. These data are representative of three biological replicates.