Fig. 10.

NAC significantly reduces the uptake of CORM-2 and CORM-3 into bacterial cells. Cultures of wild type E. coli were grown to mid log phase (OD₆₀₀ ~ 0.5) prior to the removal of 20 ml samples both before, and at regular intervals after, the addition of CO-RM. In (A) Ru uptake as CORM-3 (40 μM) in the absence (closed symbols) or presence (open symbols) of 400 μM NAC is shown. In (B) Ru uptake as CORM-2 (20 μM) in the absence (closed symbols) or presence (open symbols) of 200 μM NAC is shown. Cell pellets were assayed for Ru content by inductively coupled plasma mass spectrometry. Data are the means and standard deviations of 3 biological replicates.