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. 2013 Oct;142(4):381–404. doi: 10.1085/jgp.201311015

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© 2013 Vocke et al.

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Figure 1. — Nonstationary analysis of Ca²⁺-dependent Cl⁻ currents. (A) Whole-cell (wh.c.) recordings at −70 mV from two HEK 293 cells expressing either ANO 1 (black) or ANO 2 (red) channels. The pipette solution contained 62 µM free Ca²⁺. (B) Mean peak current densities from cells transfected with ANO 1 (252 ± 15.4 pA/pF; 24 cells) and ANO 2 (127 ± 14.6 pA/pF; 23 cells) channels. Control recordings without Ca²⁺ in the pipette solution or in mock-transfected cells yielded no detectable current. (C) Time course of channel activation with either 2.6 µM or 62 µM free Ca²⁺ in the pipette solution. ANO 1 channels activate faster than ANO 2 channels. (D) The activation time t_ON, defined as the time period from membrane disruption to 80% of the maximal current, illustrates faster Ca²⁺-dependent activation of ANO 1. Error bars indicate mean ± SEM.