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. 2013 Oct;142(4):367–380. doi: 10.1085/jgp.201310986

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© 2013 Combs et al.

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Figure 7. — Effect of SMase D on native Na_V channels in CHO cells. (A–C) Na_V currents from a CHO cell before (A) and after (B) the addition of SMase D, elicited by stepping the membrane voltage from the −100-mV holding voltage to various test potentials between −80 and 40 mV in 10-mV increments. The current traces at −20 mV from A (black) and B (maroon) are superimposed in C. Dashed lines indicate zero current levels. (D) I-V relations before (open squares) and after (closed circles) SMase D treatment; all data are presented as means ± SEM (n = 5). (E–H) Steady-state inactivation curves (E and G) and voltage dependence of inactivation time constants (F and H) from cells expressing (G and H) or not expressing (E and F) hNa_Vβ1 before (open squares) and after (closed circles) SMase D treatment; all data are presented as means ± SEM (n = 5). Curves are fits to single Boltzmann functions, yielding V_1/2 = −26.8 ± 0.4 mV and Z = 4.7 ± 0.3 (open squares), and V_1/2 = −36.8 ± 0.3 mV and Z = 4.6 ± 0.2 (closed circles) in E; and V_1/2 = −40.9 ± 0.4 mV and Z = 3.5 ± 0.2 (open squares), and V_1/2 = −53.9 ± 0.7 mV and Z = 3.6 ± 0.3 (closed circles) in G.