Table 1.
Estimates of dynamic contractile parameters from α- and β-MHC–containing fibers reconstituted with RcTnT1–43Δ and RcTnT44–73Δ
Dynamic contractile parameters | RcTnTWT | RcTnT1–43Δ | RcTnT44–73Δ |
α-MHC | |||
b (s−1) | 18.14 ± 0.62 | 18.38 ± 1.10 | 17.46 ± 0.43 |
ktr (s−1) | 7.32 ± 0.17 | 7.25 ± 0.31 | 6.79 ± 0.29 |
c (s−1) | 21.41 ± 0.83 | 20.60 ± 1.53 | 20.14 ± 0.07 |
β-MHC | |||
b (s−1) | 7.55 ± 0.34a | 7.84 ± 0.17a | 7.53 ± 0.22a |
ktr (s−1) | 4.15 ± 0.11a | 4.38 ± 0.08a | 4.40 ± 0.11a |
c (s−1) | 10.72 ± 0.40a | 10.70 ± 0.35a | 11.68 ± 0.30a |
The SL of the muscle fiber was set to 2.3 µm. b and c were estimated as described previously (Ford et al., 2010). ktr was estimated using a rapid slack/restretch protocol (Brenner and Eisenberg, 1986). Number of determinations was at least 11 for each group. Values are reported as mean ± SEM.
P < 0.001 when compared to the corresponding groups in α-MHC fibers.