Fig. 3.

Comparison of CD64 binding of surface displayed Fc-wt, original Fc-libraries and Fc-libraries after 4 rounds of sorting. (A) After induction of surface expression a heat shock was either applied (right column) or not (left column), followed by staining with CD64 and an antibody against the Xpress epitope which is used for expression normalization. For comparison, gate positions were set identically in the left and in the right column, respectively. Numbers in the dot plots indicate the percentage of cells in the gate. (B) Yeast displayed Fc-wt or Fc-libraries were incubated at the indicated temperatures for 10 min and subsequently analyzed for their binding to CD64 by flow cytometry. Mean fluorescence intensities (MFI) for CD64 binding are depicted. In A and B one representative experiment out of two is shown.