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. 2012 Oct 22;32(38):4549–4556. doi: 10.1038/onc.2012.465

Figure 3.

Figure 3

Inhibition of PHD by DMOG induces autophagy independently of HIF. U2OS cells stably expressing GFP-LC3 were transfected with a plasmid encoding control, nontargeting shRNA (shCntr) or transfected with a plasmid encoding shRNA for HIF1b (clone no. 4; shHIF1b no. 4). After 48 h, cells were incubated for 5 h in the presence of amino acids with or without DMOG as indicated. Aggregation of GFP-LC3 was analyzed by fluorescence microscopy (a) and quantified (b). Results represent the average and s.d. of three independent experiments.