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. 2013 Aug 2;12(9):4187–4192. doi: 10.1021/pr400524d

Figure 1.

Figure 1

(A) XIC of m/z = 830.3327 corresponding to monophosphorylated doubly charged Peptide 3 (SMGGGPGQSGPSYGGQR) generated in Qual Browser/Xcalibur 2.1 software (Thermo) and exported for scan-based plotting. (B) Scan-based plot of the elution of the y8-ion (m/z = 901.36) of the S355 isomer (red) and the y9-ion (m/z = 908.42) of the S344 isomer (blue) generated with the in-house developed tool (mass tolerance 0.5 Da). The elution of the two fragment ions coincides exactly with the two peaks of the parent phosphopeptide (A).