Figure 5. STAT3 is involved in regulating BACE1.

(A) pSTAT3 levels in the nucleus of primary neurons incubated in control (CM-B) or CM-P for the indicated time. Histone was used as loading control for the nuclear extracts. (B) pSTAT3 levels in the nucleus of primary neurons cultured in BSA (control) or 0.2mM palmitate (PA) for 12hr. Then the cells were lysed and western blot for pSTAT3 was performed. Histone was used as loading control for the nuclear extracts. (C, D) pSTAT3 levels in the nucleus. Primary neurons treated with control (CM-B), CM-P or CM-P plus specific inhibitors of STAT3 (STAT3Inh at 235μM or STAT3Inh VI at 100μM) for 12hr. Then the cells were lysed and pSTAT3 levels were measured by western blot and histone was used as loading control for the nuclear extracts. (E, F,G) BACE1 mRNA or protein levels in primary neurons treated with control (CM-B), CM-P or CM-P plus specific inhibitors of STAT3 (STAT3Inh at 235μM or STAT3Inh VI at 100μM) for 12hr. Then the cells were lysed and BACE1 protein levels were measured by western blotting of whole cell lysates with actin used as a loading control. The mRNA of BACE1 was detected by real-time PCR. The results were normalized to control (CM-B) and * or na (not significant) above the bar indicates the significance. A line indicates a comparison between the 2 bars connected by the line. (n=3). *p<0.05, **p<0.01, ***p<0.001.