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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Nat Genet. 2013 Jun 2;45(7):747–755. doi: 10.1038/ng.2650

Figure 3. Differential responses to castration in different genetic backgrounds.

Figure 3

(a-b) IHC staining of DLP and AP from castrated and non-castrated mice at 30 days post-castration. Upper panel: Ki-67 staining on prostate sections from castrated and noncastrated mice of indicated genotype. Lower panel: quantification of Ki-67 staining. Quantification has been performed as described in the methods. For each genotype, castrated (n=3, grey bar) and non-castrated (n=3, black bar) mice were analyzed. Percentage of Ki67 positive cells in DLP and AP prostate lobes was evaluated on a total of 15,000 cells per lobe. Data are presented as mean ± standard deviation. (c) Western blot analysis on VP, DLP and AP from castrated Ptenflox/flox;Probasin(Pb)-Cre, Ptenflox/flox;Lrfflox/flox;Probasin(Pb)-Cre and Ptenflox/flox;p53flox/flox;Probasin(Pb)-Cre mice. Western blot analyses were performed on sample lysates collected from mice sacrificed 4 days after castration. (d) IHC staining of AR on DLP and AP from castrated and noncastrated mice sacrificed 30 days after castration. (e) Quantification of cells with nuclear AR localization in castrated Ptenflox/flox;Probasin(Pb)-Cre (light grey bar), Ptenflox/flox;Lrfflox/flox;Probasin(Pb)-Cre (dark grey bar) and Ptenflox/flox;p53flox/flox;Probasin(Pb)-Cre (black bar) prostate tumors. Number of cells with nuclear AR staining in DLP and AP prostate lobes was evaluated in n=3 castrated mice/genotype on a total of 2,500 cells for each prostate lobe. Data are presented as mean ± standard deviation.