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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Nat Genet. 2013 Jun 2;45(7):747–755. doi: 10.1038/ng.2650

Figure 4. Deregulation of XAF1 and SRD5A1 levels dictates prostate cancer progression and castration resistance in mouse and human.

Figure 4

(a) Analysis of the expression profiles of Ptenflox/flox;Lrfflox/flox;Probasin(Pb)-Cre versus Ptenflox/flox;Probasin(Pb)-Cre prostates shows a significant down-regulation of Xaf1 and a concomitant up-regulation of Srd5a1. (b) Quantitative RT-PCR of Xaf1 in VP (n=3, black bars), DLP (n=3, dark grey bars) and AP (n=3, light grey bars) samples collected from Ptenflox/flox;Probasin(Pb)-Cre, Ptenflox/flox;Lrfflox/flox;Probasin(Pb)-Cre, and Ptenflox/flox;p53flox/flox;Probasin(Pb)-Cre mice. Data are presented as mean ± standard deviation. (c) IHC with anti-Xaf1 of the same samples in b. (d) Western blot analysis on the same samples in b. (e) Quantitative RT-PCR for Srd5a1 in VP (n=3, black bars), DLP (n=3, dark grey bars) and AP (n=3, light grey bars) samples collected from WT, Ptenflox/flox;Probasin(Pb)-Cre, Ptenflox/flox;Lrfflox/flox;Probasin(Pb)-Cre and Ptenflox/flox;p53flox/flox;Probasin(Pb)-Cre mouse prostates. Data are presented as mean ± standard deviation. (f) XAF1 staining and relative quantification in human prostate cancer TMA. (g) LRF and XAF1 down regulation and SRD5A1 induction in castration resistant prostate cancer xenografts (orange bars) versus castration sensitive (black bars). (h-l) XAF1, XIAP, and SRD5A1 expression levels in primary and metastatic prostate cancer compared to normal prostatic epithelium.