Table 1.
51.1 (pfu × 106) | 44/47.1 (pfu × 106) | |||
---|---|---|---|---|
| ||||
Passage No. | CEF | Vero | CEF | Vero |
1 | 3.2 | NT | 1.2 | NT |
2a | 5.0 | 5.2 | 2.4 | 6.5 |
2b | 9.0 | 9.6 | NT | NT |
3a | 13 | 16 | 1.3 | 3.9 |
3b | 6.4 | 6.5 | NT | NT |
4a | 4.8 | 5.3 | 0.9 | 1.2 |
4b | 6.2 | 5.4 | 0.6 | 1.8 |
5a | 9.5 | 8.4 | 4.6 | 6.7 |
5b | 20 | 20 | 1.1 | 5.4 |
6a | 6.2 | 12 | 2.0 | 3.8 |
6b | 6.5 | 5.6 | 1.4 | 4.1 |
7a | 5.6 | 8.0 | 3.2 | 8.0 |
7b | 8.5 | 13 | 1.6 | 2.8 |
a and b represent independent serial passages.
Passage 1: cells infected with 0.1 pfu/cell.
Passage 2: cells infected with 0.1 ml of previous.
Passage 3 – 7: cells infected with 0.05 ml of previous.
Cells harvested at 48 h after each passage.
NT: not titered