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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: Vaccine. 2013 Aug 6;31(41):4569–4577. doi: 10.1016/j.vaccine.2013.07.057

Table 1.

Stable replication of rMVAs in Vero cells

51.1 (pfu × 106) 44/47.1 (pfu × 106)

Passage No. CEF Vero CEF Vero
1 3.2 NT 1.2 NT
2a 5.0 5.2 2.4 6.5
2b 9.0 9.6 NT NT
3a 13 16 1.3 3.9
3b 6.4 6.5 NT NT
4a 4.8 5.3 0.9 1.2
4b 6.2 5.4 0.6 1.8
5a 9.5 8.4 4.6 6.7
5b 20 20 1.1 5.4
6a 6.2 12 2.0 3.8
6b 6.5 5.6 1.4 4.1
7a 5.6 8.0 3.2 8.0
7b 8.5 13 1.6 2.8

a and b represent independent serial passages.

Passage 1: cells infected with 0.1 pfu/cell.

Passage 2: cells infected with 0.1 ml of previous.

Passage 3 – 7: cells infected with 0.05 ml of previous.

Cells harvested at 48 h after each passage.

NT: not titered