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β-Elemene abrogates cisplatin-induced JNK1 protein phosphorylation in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin alone or cisplatin plus β-elemene (ELE) at the IC20 for 1 or 3 h. The cells were harvested and lysed. Cell lysates containing 60 μg of protein were analyzed on western blots using antibodies against phospho-JNK1 (Thr183/Tyr185) and β-actin (loading control), as described previously (21,22).
