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. 2013 Oct 1;8(10):e76023. doi: 10.1371/journal.pone.0076023

Figure 2. Expression of HLA-G5 and its receptors LILRB1 and KIR2DL4 in trophoblasts. (.

Figure 2

A) Western blot analysis of the total cell lysate showed HLA-G5 was expressed in human choriocarcinoma JEG-3 cells but not in JAr or human natural killer NK92mi cells. JEG-3, JAr and NK92mi cells expressed both LILRB1 and KIR2DL4 receptors. The β-actin protein was used as loading control. (B) Immunofluorescent staining (green) showed the LILRB1 and KIR2DL4 receptors were expressed in the JAr and JEG-3 cells mainly in the cytoplasm and membrane areas. Representative images are from three sets of individual experiments. (C) Human primary trophoblastic cells stained for HLA-G5, LILRB1 and KIR2DL4 receptors. The nuclei of the cells were counterstained with DAPI. 100x Magnification.