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. 2013 Oct 1;8(10):e76103. doi: 10.1371/journal.pone.0076103

Figure 6. Survival of GFP-positive rASCs after 24 weeks of implantation and their differentiation to functional keratocytes.

Figure 6

(A-H) corneas implanted with autologous rASCs-PLGA constructs; (A, E) GFP expression, (green color); (B, F) nuclei staining by hoechst33258 at the same section, (blue color); (C) expression of keratocyte specific proteoglycan keratocan at same section, (red color); (G) expression of ALDH1A1 at same section, (red color); (D, H) GFP expression, nuclei staining and keratocan, ALDH1A1 immunostaining are superimposed with Adobe Photoshop software, showing keratocyte proteoglycan production by implanted GFP-positive rASCs (Bar scales: 50 µm)..