Figure 1. Cloning strategy for the isolation of OfRyR cDNA sequence.

The thick black line indicates the full-length OfRyR cDNA. S1–S17 represent the nucleotide sequences of the overlapping cDNA clones. Six PCR fragments (S2, S6, S9, S13, S15 and S17) were amplified with degenerate primer sets. A degenerate primer coupled with a specific primer were used to obtain fragments S1, S3 and S7. And the rest fragments were amplified with specific primer sets. The 5′-end and 3′-end fragments were obtained through 5′-RACE and 3′-RACE.