A repeated measures before-after trial design was used to examine the efficacy of wheelchair tilt-in-space and recline angles on enhancing muscle and skin perfusion in people with spinal cord injury. Muscle and skin perfusion was simultaneously measured at the right and left ischial tuberosities, respectively. The example only lists muscle perfusion as an example of calculating normalized muscle perfusion. Tilt-in-space (T): 15°, 25,° 35° and recline (R): 100°, 120°. An example of a complete protocol for a research participant.