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. 2013 Oct 1;8(10):e75291. doi: 10.1371/journal.pone.0075291

Table 1. Primer and probe combinations for analysis of pollen parent of transgenic embryos.

Name Primer 1 Primer 2 Probe
Papaya ringspot virus coat protein (PRSV-CP) TCTTCAGTACAATCCGCAACA TCACTCCCTCATACCACTTCTC 56-FAM/TTTCTAACT/ZEN/CTCGTGCCACTCATTCACA/31ABkFQ
Plum pox virus (PPV)-CP TTGGTATGAAGGAGTTAAGCGAG TCCACTTGTGTTTCCCCATC 56-FAM/TGATGTCAC/ZEN/GGACGATGAAATGAGCA/31ABkFQ
HONEYSWEETPPV-CP hairpin transgene insert GGTAGTTCCCACTGAATCAAAGGC TTATTTCAACGCCAGTCCTGTCCC 56-FAM/TGGCAAGGA/ZEN/AATGTGCGAGTTCTGT/31ABkFQ
CAB Chlrophyll binding protein TCAACTGGGCCCTTTCCAGTTACA GGCGTTTGCTGAACTGAAGGTGAA 56-FAM/AATGGGCGG/ZEN/CTGGCAATGACTTCAAT/31ABkFQ