Table 1. Primer and probe combinations for analysis of pollen parent of transgenic embryos.
Name | Primer 1 | Primer 2 | Probe |
Papaya ringspot virus coat protein (PRSV-CP) | TCTTCAGTACAATCCGCAACA | TCACTCCCTCATACCACTTCTC | 56-FAM/TTTCTAACT/ZEN/CTCGTGCCACTCATTCACA/31ABkFQ |
Plum pox virus (PPV)-CP | TTGGTATGAAGGAGTTAAGCGAG | TCCACTTGTGTTTCCCCATC | 56-FAM/TGATGTCAC/ZEN/GGACGATGAAATGAGCA/31ABkFQ |
HONEYSWEETPPV-CP hairpin transgene insert | GGTAGTTCCCACTGAATCAAAGGC | TTATTTCAACGCCAGTCCTGTCCC | 56-FAM/TGGCAAGGA/ZEN/AATGTGCGAGTTCTGT/31ABkFQ |
CAB Chlrophyll binding protein | TCAACTGGGCCCTTTCCAGTTACA | GGCGTTTGCTGAACTGAAGGTGAA | 56-FAM/AATGGGCGG/ZEN/CTGGCAATGACTTCAAT/31ABkFQ |