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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Allergy Clin Immunol. 2013 Jun 24;132(4):856–865.e3. doi: 10.1016/j.jaci.2013.05.008

Figure 4. IFN-γ potentiation of expression of markers for mature eosinophils.

Figure 4

Figure 4

CD34+-enriched hematopoietic stem cells were cultured for 3 days with SCF, TPO, Flt3L, IL-3, and IL-5 after which they were cultured for 3 additional weeks with just IL-3 and IL-5 with or without the additional presence of IFN-γ. Media was changed weekly. Maturation status was assessed by flow cytometry for CCR3 and Siglec-8. A. Cells matured with IL-5 and IL-3 only. B. Cells matured in the additional presence of IFN-γ. C. Summary data for all 5 samples.