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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Int J Cancer. 2013 Jun 26;133(11):2577–2586. doi: 10.1002/ijc.28293

Figure 1. Expression of Notch3 in PC cells exposed to normoxia and hypoxia.

Figure 1

A. Total cell lysates from cells exposed to normoxia (N), normoxia + 20 μM DAPT (N+D), hypoxia (H) or hypoxia + 20 μM DAPT (H+D) for 7 days, were analyzed by Western blotting using anti-Notch3 specific antibody. FL N3: full length Notch3; GAPDH indicates normalization of protein loading. B. Total cell lysates from cells exposed to normoxia (N) or hypoxia (H) for 7 days, were analyzed by Western blotting using anti-Notch3 and anti-HIF1α specific antibodies. TM N3: transmembrane Notch3; N3 ICD: Notch3 intracellular domain. GAPDH indicates normalization of protein loading. C. Notch3 immunostaining of PC cells exposed to normoxia or hypoxia for 7 days.