a, b: mild cytoplasmic staining in normal prostatic gland; c, d: mild cytoplasmic staining in high grade PIN, more intense at the apical pole of glandular cells; e, f: moderate cytoplasmic staining in Gleason grade 3 PC; g–j: strong cytoplasmic and nuclear staining in Gleason grade 4 (g, h) and Gleason grade 5 (i, j) PC. Original magnification: a–d, 400x; e–j, 200 x.