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. 2013 Oct 1;8(10):e74717. doi: 10.1371/journal.pone.0074717

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© 2013 Margaret et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, sodium dodecyl sulphate-polyacrylamide gel electrophoresis and silver-staining or B, immuno-staining using the monoclonal antibody NB6-228.22 of lipopolysaccharides (LPS) crude extracts from Sinorhizobium fredii HH103 and its lpsB (SVQ613, SVQ615), lpsE (SVQ642, SVQ647), and greA (SVQ655, SVQ656) mutant derivatives. Lanes 1 and 8, HH103 Rif^R; lane 2, SVQ613; lane 3, SVQ615; lane 4, SVQ642; lane 5, SVQ642 C1; lane 6, SVQ647; lane 7, SVQ647 C2; lane 9, SVQ655; lane 10, SVQ655 carrying pMUS908; lane 11, SVQ656; lane 12, SVQ656 carrying pMUS908. The rough and smooth forms of the wild-type LPS are indicated as R-LPS and S-LPS, respectively. The relevant characteristics (gene mutated, cassette employed and, when necessary, orientation of the cassette) are indicated on the top of each lane. Compl. = complemented.