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. 2013 Oct 1;8(10):e76355. doi: 10.1371/journal.pone.0076355

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© 2013 Behrens et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Co-immunoprecipitation of full length FEZ1 tagged N-terminally with a V5 epitope and various EmGFP-SCOC(78-159) variants from transfected HEK 293 cell lysates using an anti-GFP antibody. (B) Co-expression of Strep-SCOC(78-159) constructs with His₆-FEZ1(227-290). Samples eluting from a StrepTrap column were analyzed with a Coomassie stained Schägger gel. (C) SEC-MALLS measurement of wild-type SCOC(78-159)-FEZ1(227-290) complex. The concentration of the complex ranged from 1 to 1.5 mg/ml for SEC-MALLS measurements.