| Strep-SCOC ccd M78 NcoI fwd |
gaattccatatgatgaatgccgacatggatg
|
| Strep-SCOC rev 1 |
ctccagctgccgcgcggcaccagtttacgtttgg
|
| Strep-SCOC XhoI rev 2 |
ccgctcgagttatttttcgaactgcgggtggctccagctg
|
| SCOC-Gateway ccd M78 fwd |
caccatgatgaatgccgacatggatgc
|
| SCOC-Gateway rev |
tttacgtttggatttggtatcggtggtttga
|
| SCOC L105M fwd |
acccgtctgatcaaccaagttatggagctgcag
|
| SCOC L105M rev |
ctgcagctccataacttggttgatcagacgggt
|
| SCOC R99E fwd |
gtggaactggaggaaaaaaccgagctgatcaaccaagttctggag
|
| SCOC R99E rev |
ctccagaacttggttgatcagctcggttttttcctccagttccac
|
| SCOC R117E fwd |
actggaagatctgtctgccgaggtcgatgccgtaaaagagg
|
| SCOC R117E rev |
cctcttttacggcatcgacctcggcagacagatcttccagt
|
| SCOC E93V fwd |
tgccgaaaatcaggtggtactggaggaaaaaaccc
|
| SCOC E93V rev |
gggttttttcctccagtaccacctgattttcggca
|
| SCOC E93V /K97L fwd |
gccgaaaatcaggtggtactggaggaattaacccgtctgatcaac
|
| SCOC E93V /K97L rev |
gttgatcagacgggttaattcctccagtaccacctgattttcggc
|
| SCOC N125L fwd |
gtgtcgatgccgtaaaagaggagctactgaaactgaaaagtgagaatca
|
| SCOC N125L rev |
tgattctcacttttcagtttcagtagctcctcttttacggcatcgacac
|
| SCOC N132V fwd |
gaatctgaaactgaaaagtgaggttcaagtgctgggccagtatatc
|
| SCOC N132V rev |
gatatactggcccagcacttgaacctcacttttcagtttcagattc
|
| FEZ1 ccd M227 NdeI fwd |
ggaattccccatatgtctgggtctgag
|
| FEZ1 ccd L290 XhoI rev |
ccgctcgaggctgccgcgcggcaccagctttttcatcagttctcgctg
|
| synthetic gene of SCOC isoform 1 full length |
atgcgtcgccgtgtgttttctagccaggattggcgtgcttcaggatgggacggtatgggcttttttagtcgtcgtaccttctgtggtcgttcaggtcgttcttgtcgtggacagctggttcaagtttctcgcccggaagtttctgctggttctctgctgctgcctgctccacaagccgaagatcatagcagtcgtattctgtatcctcgcccaaaatccctgctgccgaaaatgatgaatgccgacatggatgccgttgatgccgaaaatcaggtggaactggaggaaaaaacccgtctgatcaaccaagttctggagctgcagcatacactggaagatctgtctgcccgtgtcgatgccgtaaaagaggagaatctgaaactgaaaagtgagaatcaagtgctgggccagtatatcgaaaatctgatgagcgcctctagcgtctttcaaaccaccgataccaaatccaaacgtaaataa
|
| C-terminal Strep-tagged SCOC(78-159) construct, SCOC sequence is underlined |
MAMMNADMDAVDAENQVELEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRKLVPRGSWSHPQFEK |
