Figure 5. EW up-regulates E-cadherin expression in TCam-2 cells.

A) Western blot analysis of E-cadherin in TCam-2 cells cultured with or without 10% EW for 72 hours. As expected a 124kDa band was detected by immunoblot analysis. The densitometric analyses of the bands, normalized versus tubulin and versus GAPDH, observed in three independent experiments, are reported. Statistical significance was evaluated by Student’s T test. * vs. control, P< 0.05. B) E-cadherin immunolocalization in TCam-2 cells cultured in absence (I) or in the presence of 10% EW (III) for 72 hours. In II and IV the respective bright fields are reported. Bar, 20 µm.