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. 2013 Oct 1;8(10):e78521. doi: 10.1371/journal.pone.0078521

Figure 8. HIV-1 gp120-specific total IgG (panel A), IgG1 (panel B), IgG2a (panel C) and IgA (panel D) antibodies in vaginal washes collected from guinea pigs, detected by isotype-specific ELISA with purified gp120.

Figure 8

The guinea pigs were immunized with the indicated rNDVs by the i.n. route. Mean ELISA end-point titers of gp120-binding vaginal wash antibodies of the indicated isotype on days 0, 7, 14, 21, 28, 42, 56, 70 and 90 are shown. Antibodies specific to gp120 were not detected in any animal on any day in control rLaSota group. The graph shows the geometric mean value ± SEM for 3 animals in rLaSota, rLaSota/gp160 and rLaSota/gp140L groups, 6 animals in rLaSota/gp140S group and 5 animals in rLaSota/gp120 group. Arrows indicate time of rNDV immunizations on days 0 and 14. Statistical differences between the groups were calculated by unpaired t test (two-tailed). 1* indicates statistically significant differences (P<0.05) of rLaSota/gp140S vs. rLaSota/gp160, rLaSota/gp140L and rLaSota/gp120 groups. 2* indicates statistically significant differences (P<0.05) of rLaSota/gp140S vs. rLaSota/gp160 and rLaSota/gp140L groups.