Figure 7. Association of pH induced contractolation with expression of heat shock proteins.

Cells were lysed immediately after removal from the CO₂ incubator (0 min, pH 7.4),or after 10-60 min exposure to normal atmosphere. The pH of the medium was measured at the time of cell lysis and is indicated under each test condition. (A) pII and (B) YS1.2 cell lysate proteins (8 µg) were electrophoresed on 10% SDS polyacrylamide gel, blotted onto nitrocellulose membrane and probed with antisera to HSPs 40, 90, 60, 70, HSF1 and actin as described in Methods.