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. 2013 Aug;138(2):244–251.

Fig.

Fig.

a. PCR amplification of P1 gene fragments for positive control preparation. Lane M: 1 kbp ladder, lane 1: P1 gene 543 bp positive PCR, lane 2: no template control. b. Restriction digestion of positive clones. Lane M: 1 kbp ladder, lanes 1-4: positive clones.