Figure 2.

Ultrastructural observation of budding vesicle from exocytotic spots in stimulated chromaffin cells. To stain specifically the granule membrane fused with plasma membrane, stimulated cells are incubated in the presence of antibodies raised against the luminal region of dopamine-beta hydroxylase (DBH), a transmembrane marker of secretory granules (asterisks). Note that small vesicles budding can be observed from the DBH antibodies clusters (black arrowhead in enlarged view) suggesting a partial recapture of the granule membrane. The white arrowhead shows a granule fusing with the plasma membrane. Bar = 100 nm.