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Myristylation and ligand binding produces altered mobility of the myristate pocket. (A) Anisotropy decay of FM-N99C in myristylated/apo state (blue), nonmyristylated/apo state (red), myristylated/ternary state (orange), and nonmyristylated/ternary state (green). IRF is the instrument response function. (B–D) RMSF values of the main-chain atoms from MD simulations for residues 90–105 (B), 150–160 (C), and 300–310 (D).
