Figure 5.

Long-term maintenance of the NPC. (A) NPC pore density through aging. Brain and liver nuclei were purified from 6 week, 6, 13, and 24 month-old rats and fixed. NPCs were stained and visualized by super-resolution microscopy, and individual pores counted for each nucleus (n > 30). Plotted are the average pore densities (pores per nuclei surface area [μM]) for brain (orange) and liver (grey) nuclei across the entire time span. See also Figure S5. (B) ¹⁵N fractional abundance decay of the Nup205 complex. ¹⁵N fractional abundance was determined from multiple peptides for the indicated members of the Nup205 complex, from neuronal-enriched nuclei from all time points of the pulse-chase and plotted over time. (C) ¹⁵N fractional abundance decay of the Nup107/160 complex. ¹⁵N fractional abundance was determined from multiple peptides for the indicated members of the Nup107/160 complex, from neuronal-enriched nuclei from all time points of the pulse-chase and plotted over time. All error bars represent standard deviations.